UV spectrophotometric absorption correction method has been developed for simultaneous estimation of mefenamic acid and tranexamic acid. Wavelength selected for TRA was 402.6nm and for MAA corrected absorbance was measured by deducing the absorbance of TRA at 402.6 nm from absorbance of mixture at 297.2 nm. Overlay spectra for both the drug and selected wavelengths are shown in Figure 2. Beer’s law obeyed in concentration range of 4-20 μg/ ml MAA and 8-40 μg/ ml in methanol for both the drugs. Limit of detection was found to 0.173 and 0.346 μg/ ml and limit of quantitation 0.524 and 1.048 μg/ml of mefenamic acid and tranexamic acid respectively. The proposed methods are simple, sensitive, accurate and precise since they are recommended for routine analysis.
Loading....